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Paul Young

  Cell cycle genetics and molecular biology

  Contact Information:  
  Professor of Biology

Tel: (613) 533-6148
Fax: (613) 533-6617
email: youngpg@biology.queensu.ca

  My interests revolve broadly around cell biology and development in the fission yeast. Yeast offers the advantage of well-developed genetics and ease of cloning of genes by plasmid complementation. With the increasing resources available from genome sequencing projects, findings can often be rapidly translated to mammalian or plant systems. My major area of interest is cell cycle control. Yeast systems have led in defining the basic paradigm of eukaryotic cell cycle control (Nobel prizes to P. Nurse and L. Hartwell in 2001 for this work) and in continuing to find new elements in the puzzle. Many of the proteins discovered to play a role in yeast have their direct mammalian counterparts.

Projects in the lab focus especially on the mitotic control and the way in which external information such as nutritional status bears on its regulation. In addition we are investigating checkpoint pathways which serve to arrest major events of the cell cycle when earlier events are incomplete or not executed properly. A second area of interest is ion transport and pH regulation in cells. We have studied the sodium/protein antiporter from yeast and are currently involved in investigating related transporters and regulators. A recent interest is to examine pH regulation and its coupling (or lack of it) to the cell cycle. The projects in the lab range widely from pure genetics to molecular biology or biochemistry. We make extensive use of GFP tagged proteins and live cell imaging. The strength of a genetic system such as yeast is the ability to move easily from one approach to another and to bring all to bear on an interesting problem in biology.

  Chua, G., C. Lingner, C. Frazer and P. G. Young. 2002. The sal3 gene encodes an importin-b implicated in the nuclear import of Cdc25 in Schizosaccharomyces pombe. Genetics 162, 689-703. (pubmed link)

Karagiannis, J., R. Oulton and P. G. Young. 2002. The Scw1 RNA binding domain protein is involved in the regulation of septation and cell wall structure in fission yeast. Genetics 162, 45-58. (pubmed link)

Rupes, I. and P. G. Young. 2002. Morphogenetic checkpoint in fission yeast? No! Microbiology 148, 2271-2272. (pubmed link)

Taricani, L., M. Tejada and P. G. Young. 2002. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. 277, 10562-72. (pubmed link)

Rupes, I., B. A. Webb, A. Mak and P. G. Young. 2001. G2/M arrest caused by actin disruption is a manifestation of the cell size checkpoint in fission yeast . Mol. Biol. Cell 12, 3892-3903.

Karagiannis, J. and P. G. Young. 2001. Intracellular pH homeostasis during cell cycle progression and growth state transition in Schizosaccharomyces pombe. J. Cell Sci. 114, 2929-2941. (pubmed link)

Taricani, L., H. Feilotter, C. Weaver and P.G. Young. 2001. Expression of hsp16 in Schizosaccharomyces pombe in response to nucleotide depletion is regulated via the spc1 MAP kinase pathway. Nucleic Acids Research 29, 3030-40. (pubmed link)

Chua, G., L. Taricani, W. Stangle and P. G. Young. 2000. Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe. Nucleic Acids Research, 28(11), e53 (6 pages). (pubmed link)

Rupes, I., Z. Jia and P. G. Young. 1999. Ssp1 promotes actin depolymerization and is involved in stress response and NETO control in fission yeast. Mol. Biol. Cell 10, 1495-1510. (pubmed link)

Karagiannis, J., R. Saleki and P. G. Young. 1999. The pub1 E3 ubiquitin ligase negatively regulates leucine uptake in response to NH4+ in fission yeast. Curr.Genetics 35, 593-601. (pubmed link)

Dibrov, P., P. G. Young and L. Fliegel. 1998. Physiological consequences of expression of sod2 in Escherichia coli . Mol. Cell Biochem. 183, 125-132. (pubmed link)

Dibrov, P., P. G. Young and L. Fliegel. 1998. Functional analysis of amino acid residues essential for activity in the Na+/H+ exchanger of fission yeast. Biochemistry 37, 8282-8288. (pubmed link)

Breeding, C. S., J. Hudson, M. K. Balasubramanian, S. Hemmingsen, P. G. Young and K. L. Gould. 1998. The cdr2+ gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe. Mol. Biol. Cell 9, 3399-3415. (pubmed link)

Saleki, R., Z. Jia, J. Karagiannis and P. G. Young 1997. Low pH tolerance in Schizosaccharomyces pombe requires a functioning pub1 ubiquitin ligase. Mol. Gen. Genetics 254, 520-528. (pubmed link)

Dibrov, P., J. J.. Smith, P. G. Young and L. Fliegel 1997. Identification and localization of the sod2 gene product in Schizosaccharomyces pombe. FEBS Letts 405, 119-124. (pubmed link)

Rupes, I., J. Jochova and P. G. Young. 1997. Markers of cell polarity during and after nitrogen starvation in Schizosaccharomyces pombe. Biochem. Cell Biol. 75, 697-708. (pubmed link)

Hahnenberger, K.M., Z. Jia, L. Fliegel, S. Hemmingsen and P. G. Young. 1996. Sodium tolerance and export from yeast cells. In The Na+/H+ Exchanger. L. Fliegel, ed. Landes Co., U.S.A. (pubmed link)

Hahnenberger, K., Z. Jia and P. G. Young. 1996. Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae. Proc. Nat. Acad. Sci., U.S.A. 93, 5031-5036. (pubmed link)

Saleki, R., Young, P. G. and Lefebvre, D. 1993. Mutants affected in sodium tolerance for germination in Arabidopsis thaliana. Plant Physiology 101, 839-845.

Hudson, J.D. and P. G. Young. 1993. Sequence of the Schizosaccharomyces pombe gtp1 gene and the identification of a novel family of putative GTP binding proteins. Gene 25:191-193. (pubmed link)

Jia, Z., N. McCullough, L. Wong and P. G. Young. 1993. The amiloride resistance gene car1 of Schizosaccharomyces pombe. Mol. Gen. Genet. 241:298-304. (pubmed link)

Parker, L.L., S.A.Walker, P. G. Young and H. Piwnica-Worms. 1993. Phosphorylation and inactivation of the mitotic inhibitor wee1 by the nim1/cdr1 kinase. Nature 363:736-738. (pubmed link)

Rowley, R., S. Subramani and P. G. Young. 1992. Checkpoint controls in Schizosaccharomyces pombe. EMBO J. 11:1335-1342. (pubmed link)

Jia, Z., N. McCullough, R. Martel, S. Hemmingsen and P. G. Young. 1992. Gene amplification at a locus encoding a putative Na+/H+ antiport confers sodium/lithium tolerance in fission yeast. EMBO J. 11:1631-1640. (pubmed link)

Rowley, R., J. Hudson and P. G. Young. 1992. The wee1 protein kinase is necessary for radiation induced G2 delay. Nature 356:353-355. (pubmed link)

Hudson, J.D., H. Feilotter, C. Lingner, R. Rowley and P. G. Young. 1991. stf1: a new suppressor of the mitotic control gene, cdc25, in Schizosaccharomyces pombe. Cold Spring Harb. Symp. Quant. Biol. 56:5999-604. (pubmed link)

Feilotter, H., P. Nurse and P. G. Young. 1991. Genetic and molecular analysis of cdr1/nim1 in Schizosaccharomyces pombe. Genetics 127:309-318. (pubmed link)

Ducommun B, G. Draetta, P. Young, and D. Beach (1990) Fission yeast cdc25 is a cell-cycle regulated protein. Biochem Biophys Res Commun 167:301-309. (pubmed link)

Hudson, J., H. Feilotter and P. G. Young. 1990. Stf1: non-wee mutations epistatic to cdc25 in the fission yeast Schizosaccharomyces pombe. Genetics 126:309-315. (pubmed link)

Molz, L., R. Booher, P. G. Young and D. Beach. 1989. cdc2 and the regulation of mitosis: six interacting mcs genes. Genetics 122:773-782. (pubmed link)

Nasim, A., P. G. Young and B. Johnson, eds. 1989. The Molecular Biology of Fission Yeast. Academic Press, 470 pp.

Young, P. G. and P. A. Fantes. 1987. Schizosaccharomyces pombe mutants affected in their division response to starvation. J. Cell Sci. 88: 295-304. (pubmed link)

Querengesser, L. D. and Young, P. G. (1985). Changes in phosphoprotein pattern in Schizosaccharomyces pombe. Exptl. Cell Res. 159, 495-509. (pubmed link)

Young, P. G. and Fantes, P. A. (1984). Changed division response mutants function as allosuppressors in Growth, Cancer and the Cell Cycle (P. Skehan and S. Friedman, eds.), Humana Press, Clifton, New Jersey, p. 221-228.